Mg~ ( 2+) concentration, annealing temperature and cycle number of PCR system were optimized analyzed in their repeatability and precisions, and then a stable and convenient semi-quantitative RT-PCR system was established. 通过对PCR体系中Mg2+浓度、退火温度及循环次数的优化和对体系重复性、准确性的分析,最终建立了一个稳定、方便的半定量RT-PCR体系。
At the same time, the concentration of template DNA, annealing temperature and cycle times of PCR procedure were selected too. 同时又对DNA模板浓度,PCR扩增程序中的退火温度及循环次数进行了筛选。
Thus, Ac 1f temperature equalization spheroidizing annealing technique is developed, which acquired good spheroidizing quality, shortened productive cycle and saved energy in production test. 由此开发出Ac1f透烧加热的球化退火工艺,生产试验取得了球化质量好、生产周期短及节能的效果。
The results showed that the crystal size increases with the increase in the annealing temperature. Irreversible capacity loss at the first cycle can be decreased by the decrease of crystal size. 结果表明:随着氧化温度的升高三维网状氧化镍的晶粒尺寸增大,小的晶粒尺寸能够降低首次不可逆容量损失。
A novel method is presented to control the crystallographic orientation of the PZT thin films by modifying the coating layers in one annealing cycle of the sol-gel process. 通过改变Sol-Gel过程单次退火甩胶层数的方式,得到了一种可以简便地控制薄膜择优取向生长的新方法。
Spheroidizing annealing needs take a long time in the industrial production; this will increase the production cycle and costs. 球化退火在实际生产需要长时间的加热,使生产周期增长,并增加成本。
The multiplex amplification conditions were optimized according to the outcomes with the different primer concentration, Mg2+ concentration, DNA amount, annealing temperature and cycle times. 根据电泳结果对反应体系中的引物浓度、Mg2+浓度、DNA模板量、退火温度、循环次数等条件进行优化,以获得最佳反应条件。
The film quality and electrical properties of the PZT films increase with the decreasing of film thickness in one annealing cycle. The critical thickness is increased, and better ferroelectric properties are obtained. 研究结果表明,降低薄膜单次退火厚度有利于提高PZT薄膜的成膜质量和电性能,主要表现在薄膜的临界厚度得到了显著提高,铁电性能得到了增强。